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In about 15% of these genes, termination codons were created by adding a poly(A) tail at a spot where the underlying gene sequence did not stipulate a stop codon.
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After adding a poly-A tail to each RNA fragment by E sc h erichia coli poly-A polymerase (NEB, M0276S), reverse transcription for the polyadenylated RNA samples was performed with primers that are linked to unique barcodes.
In the proposed model, these ribosomes translate the poly-A tail of the mRNA, adding a poly-lysine tail to the newly synthesised protein.
The conversion explicitly accounted for the length of each transcript's poly(A) tail by adding a fixed length to each known length.
This method of controlling the biodegradability by adding a fatty acid group could be applied to other biodegradable polymers such as poly(butylene succinate adipate)s, ε-poly(caprolactone), and poly(lactic acid).
A model of the poly(A) tail was incorporated into the linear equation by adding a fixed length to each known length.
The impact strength of blends consisting of polystyrene (PS) and ethylene-propylene-diene rubber (EPDM) could be increased by adding a poly styrene/ethylene-propylene) (SEpoly styrene/ethylene-propylenec peroxide during reactive extruSEPn.
By adding a diblock copolymer, poly styrene-alt-maleic acid) (poly styrene-alt-maleiction uniform, highly crystalline VOHpoly styrene-alt-maleicre obtacidd, leading to a faster, more efficient, activation to the active catalyst.
This novel technique is shown on thin films of quinacridone by adding a protection layer of poly vinyl alcohol) (PVA).
Moreover, we have seen a number of works on tuning the work function of ITO by adding an electron transport layer such as ZnO [43], TiO2[44 46], Cs2CO3[44 46], and poly ethylene oxide) (PEO) [47].
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