Sentence examples for by adding a mutant from inspiring English sources
By adding a mutant α-syn (A53T) transgene to the mix, this model (hereafter referred to as AD-LBV mice) success-fully recapitulated all three major AD-LBV pathologies [ 22].
To verify the specificity of NF-κB protein binding, competition and supershift analysis was performed by adding an excess of unlabeled competitor or mutant oligonucleotides and rabbit polyclonal anti-NF-κB p65 Ab (Active Motif) to the incubation on ice for 20 minutes before the binding reaction.
The D12A/D66A-6His mutant was constructed by adding an oligonucleotide coding for six histidines at the carboxyl end of the enzyme to the plasmid containing the D12A/D66A mutation.
The clv1-1 mutant is implemented either as a loss-of-signal mutant (loss-of-signal model, Figure 1B) or by adding an interference mechanism acting on the CRN receptor pathway (interference model, Figure 1C).
However, since the LOD score is proportional to the number of individuals scored, this range can be easily increased by adding more mutant individuals if a linkage is questionable.
When we examined the number of genes and the GO terms they represent in the double mutants compared to the single mutants, we conclude that Gcn5, Mst2 and Elp3 are functionally redundant in repressing transcription because the number of terms enriched in the double mutants cannot be explained by adding the single mutants' terms together.
Interestingly, the prepore-to-pore transition and membrane perforation by the wild-type suilysin was largely prevented, in a dose-dependent manner, by adding the disulphide-locked mutant in the incubation process.
Infections were performed for each drug treatment by adding wild-type and mutant virus at an MOI of 0,5 in 100 µl of medium to untreated or treated cells.
