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The pH of the solution was then increased to 10 by adding a mixture of sodium hydroxide and hydrazine hydrate solution.
The tensile strength and elongation of the ABS composite were increased by 39%and6%6%, respectively, by adding a mixture of MNPs and CNTs with a total concentration of 2 wt%.
The 8 treatments were formulated to differ in dietary CP (14 and 16%; DM basis) and in the balance of absorbable AA achieved by changing the main protein source (MPS) of the concentrate mixtures [replacing soybean meal (SBM) with corn byproducts (CBP), dried corn distillers grains (DDG), and some corn gluten meal], and by adding a mixture of rumen-protected Lys and Met (RPLM).
RNA was isolated from frozen biopsies by adding a mixture of 1 ml Trizol (Invitrogen, Carlsbad, USA) and 10 µl β-mercaptoethanol, preheated up to 37°C.
Fra protein expression was induced with 700 µM copper sulfate, and, 24 hours later, Fra was activated by adding a mixture of Netrin A and Netrin B for 30 minutes at 27°C.
After centrifugation at 10,000×g for 10 min at 4°C, fragmented DNA in the supernatant was extracted by adding a mixture of phenol/chloroform/isoamyl alcohol (24∶24∶1) and centrifuged at 10,000×g for 10 min at 4°C.
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Similarly, an EGCG-containing lignin (CWDHP-EGCG) was prepared by adding a three-component mixture of CA (0.75 mmol), SA (0.75 mmol) and EGCG (0.5 mmol) to cell walls.
ATP hydrolysis was initiated by adding a 5-μl mixture of ATP and DNA to final concentrations of 500 μM and 1 μM, respectively and the measurements were taken at 1 point per sec resolution.
A normal lignin control (CWDHP-control) was prepared by adding a two-component equimolar mixture of CA (1.3 mmol) and SA (1.3 mmol) to cell walls.
Fe3O4 nanoparticles are prepared by adding a base to an aqueous mixture of Fe3+ and Fe2+ chloride at a 2 1 M ratio.
Iron oxide nanoparticles were prepared by adding a base to an aqueous mixture of Fe2+ and Fe3+chloride at 0.5 molar ratio.
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by incorporating a mixture
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