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mRNA and aRNA were reverse transcribed by adding a mix consisting of 8 μl 5 × First Strand Buffer (GIBCO), 2 μl Primer-Mix (oligo dT) and randomeres for mRNA or randomeres only for aRNA) (MEMOREC), 2 μl low C dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dTTP; 4 mM dCTP), 2 μl FluoroLink™ Cy3/5-dCTP (amersham pharmacia biotech), 4 μl 0,1 M DTT, and 1 μl RNasin (20 40 U) (Promega).
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This multi-line split DNA synthesis (MLSDS) is simply realized by adding a mix-and-split process to normal DNA synthesis protocol.
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A rosemary extract (Herbalox®) and crystalline ascorbic acid were the most effective antioxidants in this feed and the effect of ascorbic acid was enhanced by adding a tocopherol mix.
They have strong ambitions to win over more low-end consumers by adding a broader mix of food to its stores.
By adding a broader mix of food along with health and beauty products to its stores, could prove to be very profitable for the company.
The Mountain View-based company has ventured into the matchmaking business this week by adding a new feature called "Mix" that shows you, for the first time on Loopt, strangers in your vicinity.
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Nail trimming can be a new and scary experience for a young kitty, and you don't want to make it worse by adding a bath into the mix.
Renewable surface was proposed to enhance the evaporation by adding a collector for liquid mixing before further evaporation.
Now, by adding a third juice to the mix, the team has tested whether these neurons reflect transitivity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com