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The bottoms of the wells were sealed by adding a drop of molten agar into the wells using a sterile pipette.
The nanoporous alumina membrane was placed on the freshly cleaned, smooth Au surface and fixed by adding a drop of acetone.
For the analysis of dissolved methane, a pre-cleaned 25 ml glass vial was filled with unfiltered water, capped under water, and preserved by adding a drop of bactericide at the sampling site and kept chilled with ice until delivered to the laboratory.
After 1h and 45 min in darkness at a constant temperature of 20°C we halted the growth by adding a drop of 100% glycerol.
Gram testing was done by adding a drop of 3% w/v aqueous KOH to a sub-sample of the colony on a microscope slide and confirming the presence of Gram negative isolates by an observed increase in viscosity.
We found that SAP nanoscaffolds could be formed directly on wounded or non-wounded control HSEs by adding a drop of peptide solution directly onto the upper surface of these tissues.
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Cover the turned agar cube with the samples inside it by adding a new drop of agar.
Test for emulsification by adding a few drops of the mixture to one glass of water.
The aminoalkyl-polyfluorenes have good solubility in organic solvents and also are able to dissolve in methanol by adding a few drops of acetic acid.
This was followed by clarifying the turbid solution by adding a few drops of diethanolamine.
Soon after collection, all samples were acidified by adding a few drops of concentrated HNO3.
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