Sentence examples for by adding a bead from inspiring English sources

Total DNA/RNA were extracted from snap-frozen cecum contents using the TriPure reagent according to manufacturer's protocol, modified by adding a bead (≤ 106 μm diameter -beating step (6,500 rpm, 3 × 30 s).

DNA was extracted from frozen caecum contents using the QIAamp DNA mini stool kit (Qiagen, Courtaboeuf, France), slightly modified by adding a bead (≤106 μm diameter) beating step (6500 rpm, 3×30 s).

The simulations were performed by adding a new bead at the C-terminal of the nascent chain every 125 milliseconds.

We tested this by adding an additional bead purification step to remove to remove unligated adapters from our multiplexes of 30 32 individuals (Table 1, rows 11 13).

A cohort of 4.0×10 cells (pink bar, left) was labeled with beads, by adding a known number of beads (4.8 × 10 beads, pink bar, right ).

Translation is performed by adding a new CG bead to the C-terminus of the nascent protein and attaching it to the ribosome exit channel; the previous C-terminus is released from the exit channel.

Adjustments were made, for example, by adding a cape of bugle beads (on a Chanel piece) or raising the originally plunging neckline (on a Gaultier).

This was achieved by adding a diluted four element beads solution and then loaded into a 96-well plate for autosampler application.

RNAlater was replaced in the 2 ml plastic tube with 1.0 ml of RLT buffer (Qiagen GmbH., Germany), and the tissue was homogenized by adding a 5 mm diameter Zirconium bead (Funakoshi, Japan) and shaking with a MixerMill 300 (Qiagen GmbH., Germany) at a speed of 20 Hz for 5 min (only the outermost row of the shaker box was used).

The concentration of MVs was determined by adding a known concentration of calibrated beads (AccuCheck, Life Technologies) according to the manufacturer's protocol.

RNA extraction methods and DNase treatment were adapted from prior studies by adding an additional glass bead lysis step during the lysozyme treatment.