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Reads were discarded if they were spoiled by adaptor contamination, contained ambiguous (N) base calls, or if more than 10% of bases had quality values < 30.
Clathrin, coupled to the membrane by adaptor proteins, is thought to play a major structural role in endocytosis by self-assembling into a cage-like lattice around the forming vesicle.
This raw dataset was processed with a bioinformatics' pipeline as follows: (1) remove low quality reads; (2) trim 3' prime adaptor sequences by a modified dynamic programming algorithm; (3) remove adaptor contaminants formed by adaptor and adaptor ligation; (4) collect short RNAs ranged from 18 30 nt and draw size distribution; (5) remove those sequences with ployA tail.
Barcode sequences can be attached to genomic DNA and transcriptomes by adaptor ligation.
(i) A novel target sequencing method that adds barcode sequences by adaptor ligation.
The cellular machinery that incorporates iron-sulfur clusters into proteins is directed to particular targets by adaptor proteins.
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Four different full length genes were cloned by library screening and a fifth, a partial gene, by adaptor-anchored PCR.
AAA+ proteases are frequently regulated by adaptors that modulate spatial and temporal control of protein turnover.
The device discovery command is interpreted into other forms of broadcast messages that are meaningful in different network protocols by adaptors.
Substrate recognition by adaptors often depends on posttranslational modification of the substrate, which thus places substrate stability under dynamic regulation by intracellular signaling events.
In the population rate code, the left- and right-tuned populations contribute with opposite patterns of the adaptation effect, each being more attenuated by adaptors in their preferred location.
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