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On expression of oncogenic Ras in zebrafish larvae, we observed a robust induction and accumulation of ROS throughout the body of the animals ROS were detected in vivo by adapting a protocol based on DHE.
We tested whether repeated exposure to Tf Hp may lead to the development of resistance in treated cells by adapting a protocol used previously by us to study resistance to a ligand Ricin A conjugate (Firer et al, 2003).
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To this end, we developed a zebrafish primary hepatocyte cell culture model, adapting a protocol previously published by Collodi and colleagues [ 47].
> From all resulting predictions, we calculated different performance parameters, such as recall and precision by adapting an evaluation protocol used for the comparison of a large number of TSSs predicting methods (Abeel et al., 2009).
[ H]-Inositol labeled soluble inositol polyphosphate (IP) profiling: The soluble IP profile for B cells was determined by adapting a previously described protocol (13).
By adapting a workflow of TALEN construction protocols and mutation screening approaches for use in Platynereis, we engineered frameshift mutations in three endogenous Platynereis genes.
Viable tumor tissue slices (0.5 cm × 0.5 cm × 300 μm) were generated by adapting a well-established hippocampal slice culture protocol using a Vibratome 3000 (Vibratome, St . Louis MO, USA), which uses a vibrating blade to separate cell layers instead of cutting through tissue, allowing sectioning of soft viable tissue.
Total DNA was isolated from the flash-frozen tissue samples and their associated microbiomes by adapting an established nucleic acid extraction protocol [ 31].
Once a misbehaving node is detected, the system reacts, by adapting simple protocol parameters, to mitigate the negative effects.
The minimum inhibitory concentrations (MICs) and the minimum biofilm eradication concentrations (MBECs) were determined by adapting the protocol described for the Calgary Biofilm Device (CBD) [ 19] to the standard microtiter plates.
Fluorescence in situ hybridization was performed by adapting the zebrafish protocol (Lauter et al., 2011) to Tribolium.
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