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10 days later from initial chemical treatment, each well of 96-well plate was photographed by Acumen eX3 microplate scanner (TTP Labtech) and the number of GFP+ colonies was automatically counted by ImageXpress® Micro System (Molecular Devices).
Today's meditation features an inspiring TED talk by Acumen founder Jacqueline Novogratz.
At 96 h post transfection, the cells were staining with final concentration of 1.5 μM of propidium iodide (Invitrogen, P3566) and 10 μM of Hoechst (Invitrogen, H21486), and the survival rate of cells was detected by Acumen X3 (TTP LabTech).
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Co-morbidity problems and other complex clinical situations can be solved by clinical acumen sharpened by experience, diligent review of the literature, appropriate interactions with colleagues, and obstinate perseverance.
It is true that Aquinas attributes faith to the devils but this 'faith' amounts only to their belief that what the Church teaches is the truth, arrived at not by grace but 'forced from them' reluctantly by 'the acumen of their natural intelligence' (Summa Theologiae 2a2ae, 5, 2; O'Brien 1974, 155 & 157).
One is struck by the acumen required to bring all of this together, and also by the folksy, at times almost mundane way Mr. Spielberg and his fellow filmmakers describe it all.
It has been written by a man whose love for the sport is matched, fortunately, by his acumen as an historian and facility as a stylist.
By leveraging Acumen's funds to obtain other financing, recipients are able to magnify their impact.
His visionary zeal was generally not matched by business acumen, and he ran up debts through much of his life.
He has made money on Wall Street, as evidenced by his acumen about a nice new coffee company named Starbucks, but he made his millions "only as a hobby, babe," as he tells his wife, Cathy.
That's all well and good; however, he's got 26 engineers and all of his apps are supposed to be made by Perfect Acumen, yet his website doesn't reflect the same company.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com