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Digestion was stopped the following morning by acidifying the solution with formic acid.
By acidifying the solution, a powdery yellow precipitate of the diazonium salt was separated.
This reaction took place in buffer containing D2O at 40 °C, and after a defined time it was quenched by acidifying the solution.
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By acidifying the polysulphidic solution at pH ≈ 2, the cathodic and anodic currents disappeared (curve 3 in Figure 1), due to polysulphide disproportionation to sulphide and elemental sulphur according to equilibrium reaction (3) [30,34-39]: {S}_{mathrm{x}+1}^{2-}+{mathrm{H}}^leftrightarrows {scriptscriptstyle frac{mathrm{x}}{8}}{matH}{mathrm{S}}hrm{H}{mathrm{S}}^ (3).
DOC was determined using ICP after the removal of inorganic C through acidifying the solution, as described by Stefansson et al. [55].
Molybdenum is removed by adding sodium sulfide at 80° 85° C (175° 185° F) at a pH of 10, holding for one hour, and then acidifying the solution to pH 2.5 3 and stirring for seven to nine hours to precipitate molybdenum sulfide.
But the second big way that carbon emissions are impacting reefs is by acidifying the oceans.
It was isolated by acidifying the reaction mixture with hydrochloric acid followed by filtration.
This structural arrangement would facilitate amide deprotonation by acidifying the amide hydrogen.
In multiple wells on the plate, HCl (37.5 nmol) was added at the end of the experiment to acidify the solution by about 0.15 pH units as an acid calibration point, and data were converted to pmol H+⋅min−1 per μg protein.
Finally, 8 µL of formic acid (5%) was added to acidify the solution.
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