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Our approach was validated by accurate quantification of the knockdown potency of over 250 single shRNAmiRs.
The validity of the assays was confirmed by accurate quantification of viral copy numbers (one copy per cell) from SiHa cell line, which is known to contain one HPV-16 copy or two per cell.
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By contrast, accurate quantification of osteopontin, KC and MCP-1 did not show any difference in secretion by MB49 and MB49-I, and thus did not confirm the less than twofold difference observed in the antibody array experiment (Fig 4B).
Our previous works as well as these by others showed accurate quantification of liver enzymes and mAb, using protein calibrator and SIL-peptide I.S.
As response factors measured with available standards for a subset of TAGs varied by up to 50%, accurate quantification of all TAGs was not possible.
The present study demonstrates the value of whole-genome transcriptome analysis generated by RNA-Seq for accurate quantification of gene expression on a genome-wide scale.
Using a normal-phase column for lipid separation, the multi-stage LC-MRM increased MS signals of PC species by over 10 fold, allowing accurate quantification of most PC and PE species in a single LC-MS run.
Large genomic laboratories may want to use PRT as an easy method of replicating high-throughput data by an alternative method with accurate quantification of copy number.
The reason that it might be more appropriate is that this approach does not allow a voxel-by-voxel analysis but allows a more accurate quantification of global tumor characteristics such as SUVmax.
The calibration curves were used by Quantity One™ software to generate accurate quantification of rutin, quercetin, ellagic acid, and myricetin in the experimental samples.
We demonstrate accurate quantification of BR accumulation by ratiometric analysis of BR (Amax 568) and a membrane-bound cytochrome (Amax 410).
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