Plasmolyzed plant cells colonized by C. lentis show a weak interaction between the biotrophic hyphae and the plasma membrane [ 5].
The M-site is coordinated by H242, H244, and a weak interaction with the thioether of M314, which nevertheless plays a critical role in catalysis, because mutation to His, Cys, or Asp results in an ∼95% loss of activity.
This particular area, marked with (B) in Figure 5c, resembles the situation of a nanotube with a weak interaction strength by its substrate, although only a part of the upper wall is without a direct support.
A weak interaction caused by the involvement of only one EF-hand may explain why the GST pull-down assay showed no interaction between CaM34 and GST-1C1b.
Given the recessive nature of the resistance, this could be explained by a weaker interaction between host factor(s) and viral proteins.
Partial resistance in the Cvi-1 accession can be potentially explained by a weaker interaction between Cvi-cPGK2 and PPV protein(s) as a result of the identified S78G mutation.
Based on the in vitro transcription assays, we conclude that the adverse effect of the R60E mutation on trxB transcription is likely caused by either a weaker interaction of the mutant Spx with the trxB promoter, RNAP, or both.
The rolling governed by a weak molecular interaction is a prerequisite for a stronger molecular interaction, namely adherence.
The widely used analgesic acetaminophen (paracetamol) was also found to bind the CBP bromodomain, probably by a weak nonspecific interaction.
