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The PV voltage is measured by a voltage sensor module.
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The Ca2+-activated K+ channel, Slo1, has an unusually large conductance and contains a voltage sensor and multiple chemical sensors.
Mihailescu, M. et al. Structural interactions of a voltage sensor toxin with lipid membranes.
The DHPR is located in the T-tubule membrane acting as a voltage sensor.
The output voltage is measured by a LEM Hall voltage sensor and modulated by an output voltage modulating circuit.
HCN channels are set apart from other members of this family by their unusual activation process that includes principal gating by membrane hyperpolarization (conferred by a transmembrane voltage sensor) and modulation of the voltage-dependence of activation by binding of cyclic nucleotides to the C-terminal cyclic nucleotide-binding domain (CNBD).
In the first gating model developed by Colombini and co-workers, the N-terminus is part of a voltage-sensor domain that slides in and out of the channel upon voltage gating.
β-Toxins induce a hyperpolarizing shift of the voltage-dependence of activation via a voltage-sensor trapping mechanism, whereby toxin-mediated interactions stabilize a persistently-activated conformation of the channel domain II voltage sensor [6,7].
The KCNQ1 consists of a cytosolic N-terminal domain followed by the S1-S4 voltage sensor, a canonical pore (S5-P-S6) domain, and a long cytosolic C-terminus.
Hence additional domains of α1S and/or other DHPR subunits appear to be engaged by the voltage sensor and contribute to an EC coupling signal.
Our first goal was to validate that ArcLight fluorescence changes are driven by voltage sensor conformational changes, rather than by a different mechanism.
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