PTEN expression, detected with the primary antibody anti-PTEN (clone 28H6, NeoMarkers, Fremont, CA 94539TS 106) was quantified by using a visual grading system based on the intensity of staining and the percentage of positive nuclei calculated counting of 1000 cells in 3 different optical fields at 40× magnification.
Reactivity for all antibodies was quantified by use of a visual grading system based on the intensity of cytoplasm staining (0 2) as follows: grade 0: no immunoreactivity, grade 1: if any specific staining, slightly stronger than background staining was detected, and grade 2: if clear immunoreactivity in more than half of the cancer cells were detected.
First, we classified the specimens stained by anti-MK antibody with a visual grading system employed for immunohistochemical evaluation (Moon et al, 2003).
Meller et al. [ 27] proposed a visual grading scale, used by four other studies [ 31, 32, 38, 39], where large vessel 18F-FDG uptake was compared with that of the liver.
This investigation uses a visual grading characteristics (VGC) analysis to test the validity of this recommendation.
As previously described by Havenith et al (1988) and ten Velde et al (1991), the expression of BM components was quantified using a visual grading system based on the percentage of epithelial cell lining.
The clotting score is a visual grade of dialyser and venous chamber clotting (Table 1).
For both regimens, the appearance of facial fine lines and wrinkles continued to improve from 8 to 24 weeks as measured by both visual grading and image analysis (P<0·01 for both).
After 8 weeks of treatment, the appearance of facial fine lines and wrinkles improved in both groups as measured by expert visual grading of the REAL images (P=0·05, tretinoin regimen; P<0·01, NPP regimen).
