For α-SMA staining, sections were incubated with a mouse anti-αSMA monoclonal antibody (DAKO; 1/100) followed by a donkey antimouse Cy2 polyclonal antibody diluted 1/200 by using a MOM kit (Vector) according to the manufacturer instructions.
SVM-RFE first removes the smallest sorting coefficient feature which is constructed by the weight vector according to the SVM training result, then the rest features will be retrained through SVM to obtain the new sorting coefficient.
Transductions of SPC-A-1sci and SPC-A-1 cells were performed with the aforementioned lentiviral vector according to instructions supplied by Addgene http://www.addgene.org and stable transfectants were further isolated by cell sorting (Epics Altra, Beckman Coulter) on the basis of their EGFP expression.
Each sensor obtains a noisy observation vector of the non-random parameter vector according to a linear regression model.
The bar gene of pCambia3301, the gfp gene of pCambia1302, the badh gene of Atriplex hortensis, and the ifs gene of Medicago were amplified with the primers listed in Table S2, and ligated to pDonor 221 by the BP clonase or pCR®8/GW/TOPO® vector according to the manufacturer's instructions (Invitrogen), resulting in pENTR-bar, pENTR-gfp, pENTR-badh, and pENTR-ifs, respectively.
In this paper, we make the following concrete contributions: (i) We build indexes of bus journey data by bit-vectors according to the application scenarios, taking into account particularly the temporal and spatial factors.
The "offspring" network was then tested for stability by iterating random initial vectors according to Equation 1.
A stable GL26 cell line expressing enhanced green fluorescent protein (EGFP) was obtained by a lentiviral vector transfection according to the manufacturer instructions (ViraPower Lentiviral Expression System, Life technologies, Zug, Switzerland).
This is followed by epidemiological investigation and a vector control program according to National guidelines when indicated based on serologic, virologic or epidemiological confirmation of dengue [ 33, 34].
I think you can capture a thought by a vector".
