The content of six phenolic compounds -catechin, caffeic acid, ferulic acid, hyperoside, rutin and ellagic acid) were quantified by using an Agilent Technologies 1260 series liquid chromatograph (RP-HPLC) coupled with a variable wavelength detector.
LC MS analysis was performed as described by Huang et al. The HPLC system consisted of a quaternary pump and a variable wavelength detector, all from Agilent 1100 (Bruker Daltonics, Bremen, Germany).
The derivatized putrescine was detected at a wavelength of 230 nm using a variable wavelength detector (G1314A, Agilent Technologies).
PPA and PLA were measured by HPLC (Agilent 1100 series, Hewlett-Packard, USA) equipped with an Agilent Zorbax SB-C18 column (150×4.6 mm, 5 µm) and a variable-wavelength detector at 210 nm.
It consisted of a quaternary pump, a degasser, an automatic sample injection, and a variable-wavelength UV Vis detector.
Molecular weight: Estimated by size exclusion chromatography against polystyrene standards in DMF/0.01% LiBr with a VWD (variable wavelength detector) at 250 nm.
The chromatographic apparatus consisted of a model 515 pump, a model 717 Plus autoinjector, and a model 2487 variable wavelength UV detector (Waters Instruments, USA) controlled by Empower software.
The beamline is capable of mimicking the ESS pulse structure by a double chopper system and provides variable wavelength resolution as low as 0.5% over a wide wavelength band between 1.6 Å and 10 Å by a dedicated wavelength frame multiplication (WFM) chopper system.
Andrographolide content in the samples was detected and quantified by HPLC system, supplied by Agilent Technologies comprising 1100/1200 Column Thermostat, 1200 Variable Wavelength Detector, 1100/1200 Quaternary Pump and 100/1200 Thermostatted Autosampler.
