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The difference in responses is statistically significant at the 1% level as indicated by a two sample t-test.
The first was the identification of significant differentially expressed genes by comparing FH patients and normal controls in the training set by a two sample t-test.
The ratios were compared in each case by a two sample t-test of log abundance ratios to the pool; proteins identified in all triplicate samples for the respective comparison and having a t-test p-value < 0.05 and a fold change > 1.2 or < 0.83 were regarded as being differentially expressed across the time course.
The significance of comparison among diversity indexes were accessed by a two sample z-test for comparison between means [ 78]. fru C3 exon and I3 intron haplotype networks were inferred by TCS v 1.21 software [ 79] using statistical parsimony with a 95% connection significance [ 80], and was manually converted to Newick tree format for some of the neutrality tests.
The nucleocyclic TSSs also showed a significantly asymmetric distribution of each mononucleotide around the TSSs (Fig. 2d), average A/T nucleotide incidences upstream of the TSSs were significantly higher than the incidences downstream (p < 10−116 for A and p < 10−68 for T by a two sample z-test described in Methods).
The genes in the final dataset were ordered by p-value in a two sample equal variance t-test.
We tested whether consultation to mail out intervals were associated with response to questionnaires by using a two sample t test.
Assuming normality, intracellular IDO expression was given by a one-tailed two sampled t-test comparing MFIIDO and MFIIsotype control, where MFI is the Mean Fluorescence Intensity.
Assuming normality, HLA-A2 expression was given by a one-tailed two sampled t-test comparing MFIHLA-A2 and MFIIsotype control, where MFI is the Mean Fluorescence Intensity.
This work suggests that, on an individual basis, blood-Hg levels from separate exposure time periods do not provide a reliable estimate of longer term exposure as represented by a three sample average.
P-values were calculated by conducting a one sample t-test on the adjusted intensity data of the entire array of selected hybridizations at each time point.
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