This evolution was coupled with the variation of the electrical resistivity, measured by a four probe method.
The values of surface electrical resistance of the films were recorded by a Keithley four probe Electrometer with a four-point probe head at room temperature.
In the final annotation of the genome (June 2006, http://bioinformatics.psb.ugent.be/blast/public/?project=ostreococcus), 6369 genes were represented by at least one probe (5435 by a single probe, 791 by two probes, 116 by 3 probes and 27 by more than 4 probes) but 565 oligonucleotides did not match the genome anymore in BlastN.
The analyses focused on the responses elicited by the two probe locations in each adaptation condition.
The current response was monitored by two probe measurements placed on the sensor device with the data acquisition system.
However, we excluded probe sets for five genes each recognized by two probe sets.
The expression level of ACE2 is detected by two probe sets (219962_at and 222257_s_at) in the Affymetrix microarray platform U133A (GPL96).
Most of the genes (301) are represented by a unique probe, 22 genes by two probes, 3 genes by 3 probes and one gene by 5 probes.
The SPPs were filtered to allow zero missing and zero inconsistent data, coverage by a minimum of two probes at the interrogated position, a minimum SPP width of 4 bp and a SFPdev ratio of 1.2.
MLPA is limited to a maximum of 50 probes, including reference probes, and ideally each important genomic region is covered by a minimum of two probes.
Previous versions either did not cover every region by a minimum of two probes, contained probes not working in FFPE derived DNA, or contained probes which failed for another reason.
