Sentence examples for by a transient dimerization from inspiring English sources
Stopped-flow fluorescence resonance energy transfer experiments further suggested that its folding was likely facilitated by a transient dimerization process.
Thus activation requires a transient dimerization (Mapelli et al, 2007; Yang et al, 2008) that occurs at the unattached kinetochore, in which Mad2 is in the closed form bound to Mad1 (Mapelli et al, 2006).
A second, and quite unexpected, finding is that IP6 can activate Btk strongly by binding to a newly identified site on the PH domain, probably by promoting transient dimerization of the PH-TH module.
Because IP6 activates both kinases when they are linked to the Btk PH-TH module, we propose that IP6 promotes transphosphorylation of the attached kinase by inducing transient dimerization.
Furthermore, rhodopsins on artificial membrane assemble by transient dimerization [19].
The molecular mechanism by which IP6 causes transient dimerization of Btk remains puzzling because we do not detect dimers even at concentrations as high as 30 μM.
This transient dimerization was observed in living cells by Shah and colleagues who demonstrated that only a proportion (∼50%) turned over at kinetochores and that the remainder was stable, presumably bound to stable Mad1 (Shah et al, 2004).
IP6-triggered transient dimerization of the PH domain may provide a membrane-independent mechanism for activating Btk in the nucleus or cytoplasm or may have a role in feedback stimulation of Btk that has been activated by PIP3 at the membrane.
A key question concerns the mechanism by which channel activity is disrupted by inhibiting the interaction of the dimerization motif to the dimerization pocket on an adjacent TRPM6 kinase domain.
The possibility of transient dimerization of monomeric epimerase during catalysis was excluded by SE-HPLC in the presence of excess substrate and NAD.
This activation is a consequence of a transient PH-TH dimerization induced by IP6, which promotes transphosphorylation of the kinase domains.
