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For each trait, areas were calculated by a threshold method in which the image was first converted to black and white and values of intensity under or above user-established threshold values were chosen.
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The sampling rate was 30 kHz and the action potentials were detected by a thresholding method.
Volume viewer software, which represents the volume of interest (VOI) automatically delimited by an isocontour threshold method based on the SUV, was used to calculate metabolic parameters including SUVmax, average SUV (SUVavg), MTV and TLG.
Every time a spike was detected by the threshold method, a 1.6 ms snippet was stored and used for subsequent evaluation of the clusters as well as adjustments needed for spike sorting.
A mix peak detection method is utilized to find the position of a pulse by combining threshold method and neighbor comparison method.
E.g., if one of the supra-threshold synapses had a fluorophore AFP, and the other had a fluorophore BFP, such voxel would be identified by the threshold method as a "false" synapse tagged with AFP and BFP together, even though no such synapse existed in reality.
First a background subtraction was applied and the body was extracted by a classic thresholding method.
Finally, binary interaction networks are constructed by a simple thresholding method.
The fluorescence signals were quantified by a comparative cycle threshold method.
The first step of the process (split point 1 in Fig. 2) divides the images into these ROI and the background by applying an automatic threshold method on each filtered layer.
A key conclusion is that retardation due to sorption predicted by using the continuous distribution is larger than the retardation predicted by the threshold method.
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