Sentence examples for by a third step from inspiring English sources

Furthermore, when the standard two-step procedure for antibody purification as performed by the manufacturer (based on ammonium sulfate precipitation and ion exchange chromatography) was extended by a third step of hydrophobic interaction chromatography, the "IFN-like" activity was lost and the neutralizing capacity of the respective antibodies prevailed (Fig. 1A C).

This results in a two- or more)stwo- orluation, where first the DYD or deregressed EBV are estimored ustep a traditional pevaluationsed evaluation, and second, genomic estimates of breeding values (GEBV) are determined, where may be firstwed by a therDYDtep where the traditioral EBV anderegressedwEBVhed are combinestimated[ 2].

Claimants can pursue a third step by trying to show they suffered greater psychological damage.

The unknown conditional expectations and densities are estimated by a first step kernel estimator.

The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare).

Ozone was used to remove most of the coke, followed by a second step with H2 or He.

The pellet was washed with 35 ml 1 PBS, followed by a second step of ultracentrifugation at 100,000 × g for 2 h at 4 °C.

The exosomes pellet was washed with 24 ml PBS, followed by a second step of ultracentrifugation at 100,000 × g for 1.5 h at 4 °C.

The selected in-situ regeneration consists of entering firstly steam at relatively low temperature (< 250 °C), against most of published studies, followed by a second step with air.

To obtain stringent recovery of the antibodies, a two-step affinity chromatography principle was devised to first deplete the affinity tag-specific antibodies followed by a second step for affinity capture of the target protein-specific antibodies.

The first one is a direct one-step strategy, starting with both monomers mixed in the feed, while the two-steps strategy consists in a first step of ε-CL homopolymerization followed by a second step with ε-TCL addition in the medium and its polymerization from the previously formed PCL chains.