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The resolution and statistical support manifested in RPB1 and RPB2 phylogenies, as compared to that afforded by the best available alternative, 18S rDNA, is shown by a third analysis we present here.
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This selection of materials has been guided by a first analysis of the feasibility of the device in terms of fabrication constraints.
This interpretation was supported by a second analysis the team did with another coarse division: animals with and without the sophisticated physiology, like circulatory systems and gills, necessary to buffer them from changes in the make-up of seawater.
This view is supported by a second analysis conducted in that study [18]: when they compared the signal from the amygdala and OFC with individual participant ratings, they found a significant covariation for the amygdala, but not for the OFC.
These findings were confirmed by a second analysis of protein extracts from appressorium-like structures [ 17].
The presence and localisation of the alteration was confirmed by a second analysis by Next-Generation Sequencing.
An intent-to-treat analysis is performed, followed by a second analysis with only those patients who had completed the study i.e. 1 year of follow-up.
In fact, Viridiplantae and Thaumarchaeota did not cluster together, which was confirmed by a second analysis based on a more restricted sequence sampling.
More than 83% (10/12) of discordances were attributable to PCR-RFLP, as revealed by a second analysis of the same sample.
All longitudinal discordant results were analysed twice, to confirm the tropism switch; similarly, all samples with an false positive rate from 10%to30%0% were confirmed by a second analysis starting from the amplification of the sample.
The approximate Bayesian computation approach was used on 16 distinct demographic scenarios (Additional file 2: Figure S1A and S1B), followed by a second analysis with only three demographic scenarios that presented the highest posterior probabilities (PP) in the first run [ 67, 68].
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