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IA could be isolated in inactivation protocols by a test pulse to 0 mV, because it activated at slightly more negative potentials than IK.
The total current was recorded with a 700 ms prepulse to −120 mV followed by a test pulse (40 ms, −10 mV).
The TTX-R sodium current (predominantly mediated by NaV1.8) was recorded with a 700 ms prepulse to −50 mV followed by a test pulse (40 ms, −10 mV).
The voltage dependence of steady-state inactivation was estimated using 15 s prepulses to varying potentials followed by a test pulse to +40 mV to measure channel availability (h).
The transient current IA was determined in steady-state inactivation protocols by a test pulse to 0 mV, because it is activated at slightly more negative potentials than IK.
Steady-state inactivation of Kv currents was determined via hyperpolarizing prepulses increasing in 10 mV increments from −130 mV to 50 mV (500 ms) followed by a test pulse to 50 mV (300 ms).
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Analog outputs by injecting a test pulse have been obtained from 991 pixels out of 1024 pixels.
The time course of recovery from inactivation was investigated using a 50ms conditioning pulse (−10 mV) to induce inactivation followed by a test pulses after returning to −100 mV for varying time periods to allow time for channels to transition out of the inactivated state.
DOI: http://dx.doi.org/10.7554/eLife.12088.008 The effect of the +5 mmHg prepulse on the overall current-pressure relationship was striking: When preceded by a +5 mmHg prepulse, currents elicited by a subsequent test pulse were greatly increased in amplitude.
Steady-state inactivation was determined with a double pulse protocol consisting of 500 ms prepulses from the holding potential of −120mV (or −140mV for R1632H only) to voltages between −140 and −20 mV (or −160 and −20mV for R1632H only), followed by a constant test pulse of 20 ms duration to −20 mV at a pulsing frequency of 0.5 Hz.
Nav1.8 current was elicited by a 100-ms test pulse to 0 from a holding potential of −60 mV in a small-sized DRG neuron which exposed to the bath solution with 500 nmol/L TTX.
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