Sentence examples for by a temperature sensitive from inspiring English sources

A Francisella-specific Tn5 transposon system has been developed by our group that is delivered by a temperature sensitive plasmid [35].

We previously reported that stable expression of Notch intra cellular domain (ICN) suppresses transformation of embryonic quail neuroretina (QNR) cells induced by a temperature sensitive v-Src (QNR/v-srcts), without altering oncoprotein expression nor its downstream signaling activity.

TARGET regulates GAL4 expression by a temperature sensitive GAL80ts element, which can be expressed and repressed at 18°C and 30°C, respectively (McGuire et al., 2004).

The requirement for synaptic activity in maturing PAM neurons was tested directly by expression of a temperature sensitive mutant of the dynamin orthologue, shibire ts (Shi ts ).

Synaptic release in MB neurons of adult flies was inhibited by expression of a temperature sensitive dominant negative dynamin transgene (UAS-Shi ts ) under control of either mb186bGAL4 or mb247GAL4 drivers.

In this approach, Pxn-GAL4 mediated induction is controlled by ubiquitous expression of a temperature sensitive variant of yeast GAL80 (GAL80ts).

To prevent any transgenerational contamination by self-progeny production, a temperature sensitive sterile strain [TJ1060 spe-9 hc88 I; spe-9 hc88 II] (Fabian & Johnson, 1994) was used.

E. coli DB3.1 was co-transformed with pWY189 and pKD46 which encodes the bacteriophage lambda Red homologous recombination functions regulated by arabinose induction and possesses a temperature sensitive origin of replication [ 41].

Mutants F-P2, AX-H9, CF-B19, and FE-N3 were generated by Gubbels and screened for a temperature sensitive growth defect (permissive and restrictive temperatures were 35°C and 40°C, respectively) [ 8]; mutants SBR1-3 were generated by the Blader lab and screened for resistance against the kinase inhibitor SB505124 [ 33].

To understand the mechanisms by which Notch signaling activation suppressed cell transformation, we compared the transcription profile of QNR cells transformed by a v-src mutant encoding a temperature sensitive (ts) oncoprotein (QNR/v-srcts), with that of cells stably expressing ICN (QNR/v-srcts/ICN).

Others involve pumping of a temperature sensitive sol-gel, followed by induced gel formation via temperature change [ 1].