The dissociation was stopped by adding an equal volume of stop buffer (250 U/ml DNAse I (Sigma), 0.2% BSA (Sigma), 10 μg/ml gentamicin (Sigma) and 20 mM HEPES (Sigma), pH 7.4 in HBSS)) for 15 min at 37 °C.
Nuclei (0.1 ml) were digested with micrococcal nuclease (10 units, Worthington Biochemical Co). by incubation for 7 min at 37°C and 400 μg RNase A (Qiagen, Vinlo, Limberg, Germany) was added and digestion was continued for an additional 3 min. Digestion was stopped by adding an equal volume of 2X stop buffer (20 mM Tris. Cl, pH 7.5, 200 mM NaCl, 2% SDS and 20 mM EDTA).
The reactions were stopped by adding an equal volume of the STOP solution: 95 % formamide and 100 m m EDTA.
The reaction was stopped by an addition of equal volume of 2× SDS sample loading buffer followed by incubation for 10 min at 95°C.
500 µl TMB soluble was added and the reaction stopped by an equal volume of 1M HCl after 5 min at RT. 100 µl of each solution was transferred to Maxisorp ELISA strips (Nunc, Roskilde, Denmark) and the absorbance measured at A450 nm.
After washing in PBST, 500 µl TMB soluble was added and the reaction stopped by an equal volume of 1M HCl after 5 min at RT. 100 µl of each solution was transferred to Maxisorp ELISA strips and the absorbance measured at A450 nm.
The reaction was stopped by adding an equal volume of acidic (2N HCl) stop solution.
He controlled the race despite a scare caused by a pit-stop mix-up to equal a record of nine consecutive F1 victories set by Alberto Ascari in 1953.
But first, a stop by the Boardwalk.
Digestion was stopped by addition of an equal volume of stop-lysis buffer (50 mM Tris HCl, 600 mM NaCl, 100 mM EDTA, 1 % SDS, pH 7.9).
