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During stimulation, a 0.5-s air pulse (1.0 L min−1) controlled by a stimulus controller (CS-55; Syntech) was passed through the stimulus pipette, which was inserted into a hole in the glass tube.
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The conditioning chamber was interfaced to a stimulus controller (Coulbourn Instruments, Lehigh Valley, PA).
Stimuli of 500 ms were presented in ascending order of dosage with 30 s inter-stimuli intervals, using a stimulus controller (CS-55, Syntech).
For odorant application, a stimulus controller was used (Stimulus Controller CS-55, Syntech, Hilversum, The Netherlands).
A stimulus controller (CS-55, Syntech) delivered odor stimuli to the bee's antennae without changing the total air flux to avoid mechanical stimuli.
Stimuli pipettes inserted inside the tube delivered odorants at a flow of 0.75 L.min−1 for 1 s using a stimulus controller (Syntech).
Using a stimulus controller (Syntech, Germany), a 0.5-s air-puff was passed through the stimulus pipette into the continuous air-stream through a hole in the glass tube at 10 cm distance from the preparation.
One bee at a time was placed into the constant airstream produced by the stimulus controller, and left for 10 s to familiarise itself with the experimental context.
A 500 ms pulse (5 ml/s) was delivered by stimulus controller CS-55 (Syntech, Germany) to deliver chemical stimulants to a humidified continuous air flow (10 ml/s) over the EAG preparation.
For the experimental procedure, the harnessed bees were put in front of an olfactory stimulus controller, which delivered a clean airstream through a syringe.
Airflow through the chamber and into the Y-tube was generated with a Syntech Stimulus Controller CS-55 set at its lowest setting.
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