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The spliceosome can be assembled in vitro by a stepwise assembly of a number of snRNPs and additional proteins on exogenously added pre-mRNA.
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Trokter et al (2012) previously succeeded in producing a recombinant human dynein complex by expressing and purifying individual components and establishing a stepwise assembly protocol.
To introduce the new restriction sites (italicized) in the MCS downstream of PTac in pUADE, a stepwise assembly of an initial construct with these new sites was performed followed by its introduction in pUADE.
A stepwise assembly through the polyacrylamide gel was observed between the monomer to dimer and finally to the fully assembled trimer band indicating the formation of 3WJs.
The CdS/MoS2 photocathode was prepared by the stepwise assembly of MoS2 and CdS quantum dots (QDs) on indium tin oxide (ITO), and achieved about 280% increasing of photocurrent compared to pure CdS QDs electrode due to the formation of heterostructure.
A novel stepwise assembly method was used to generate unique responses to two different targets sequentially.
Then as the polymerase proceeds to elongate through the gene body (GB), intronic RNA, which often constitutes the majority of the primary transcript in mammalian genes, is removed by a splicing mechanism involving the stepwise assembly of a complex set of small RNA (snRNA) and associated proteins that together make up the spliceosome (Wahl et al., 2009).
In contrast, Slx9 mediates a non-canonical stepwise assembly of a Crm1-export complex.
Furthermore, we demonstrate that RNase P disengages from rDNA in mitosis and reassociates with it at G1 phase through a dynamic and stepwise assembly process.
An illustrative example with well-documented stepwise assembly of a multiprotein is given by the assembly of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, which is a molecular engine that drives membrane fusion.
Slx9 promotes stepwise assembly of a Crm1-export complex on the NES of Rio2.
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