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Lower panels: corresponding representative I KSper elicited by a step protocol from a holding potential of −80 mV to +120 mV with 20 mV increments.
From a holding potential of −80 mV, sodium currents were inactivated by a 10 ms step to −40 mV and potassium currents were activated by a step protocol ranging from −120 to 40 mV for 2 s.
Participants completed a three-minute warm-up (15 W) followed by a step protocol starting at 25 W and increasing by 10 W−1 (pedal cadence was maintained at 50 RPM).
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Outward rectification of whole-cell currents is a hallmark of VRAC that can be better characterized by a voltage step protocol reported before [12], [26].
(A ) Different kinetic properties in the wild-type (WT) and Q672K mutant channel revealed by a voltage step protocol (750 ms in 15 mV increments).
In this study, vaccine was applied by a two-step protocol that included a single CSSS procedure on the upper arm followed by topical application of a total dose of a conventional influenza/tetanus vaccine.
The overexpressed protein was purified by a single-step protocol consisting of a strong anion-exchange column (MonoQ HR16/10).
If the three modalities of clinical presentation, exercise MRIs, and ICP measurements had already been established as a step by step protocol, the diagnosis of CECS would have been accomplished much sooner.
A step by step protocol for the allelic exchange procedure is listed under the protocols section of this work.
The denatured protein was refolded by a two-step protocol, the first step being a rapid fivefold dilution of the protein solution in 6 M GuHCl in ice-cold water.
The untagged protein was purified by a two-step protocol including cation exchange chromatography (CEC) followed by gel filtration chromatography (GFC).
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