Sentence examples for by a step gradient from inspiring English sources
The target protein binds at specific salt conditions and can be eluted by a step gradient decreasing the pH value and reducing the ionic strength.
The bound proteins were eluted by a step gradient of increasing NaCl.
Cyt b5 was then purified on a HiPrep DEAE Sepharose FF column (GE Healthcare) and eluted by a step gradient of NaCl.
The lysate was loaded onto a column containing SP-Sepharose Fast Flow resin (GE Healthcare), pre-equilibrated in 50 mM sodium acetate (pH 4.5), and ubiquitin was eluted by applying a step gradient with 50 mM sodium acetate (pH 4.5) and 1 M NaCl.
Liposome-associated and liposome free proteins were separated by flotation through a step gradient of Nycodenz medium (Axis Shield, Norway) in 20 mM Hepes/NaOH pH 7.5, 150 mM NaCl.
Viruses were purified using two consecutive CsCl gradients (a step gradient followed by an equilibrium gradient [21]) followed by elution on a Sephadex PD-10 desalting column (Amersham Biosciences, Uppsala, Sweden).
Lysates were cleared by centrifugation (15 min at 13200 rpm) and 1 ml was loaded on the top of a step gradient obtained by layering 2 ml of each of the following sucrose solutions: 40 - 32.5 – 25 - 17.5 and 10% sucrose in 50 mM Tris/Hcl pH 8.0, 150 mM NaCl, 1% CHAPS, 2 mM EGTA, 1 mM DTT.
Highly concentrated samples were fractionated by strong cation exchange chromatography in a step gradient [ 14]; all samples were desalted using STAGE Tips [ 15] before injected into a nanoflow liquid chromatography system with C18 reversed phase material and sprayed into the mass spectrometer as described in [ 5].
A step gradient was prepared by overlaying with 3 ml of 35% sucrose in TBS, followed by 0.5 ml of 5% sucrose in TBS as described in [ 25].
The residue was purified by chromatography using a stepped gradient of 0 15% MeOH in DCM.
The residue was purified by chromatography using a stepped gradient of 5 10% NEt3 in EtOAc, then 1 10 89 MeOH/NEto/EtOAc to give 43 mg of 6 as a yellow oil.
