For each sample, a one-dimensional ZGPR and NOESY experiment (referred to as 1D-NOESY), including water signal saturation during relaxation, mixing time, and a spoil gradient, was performed.
A one-dimensional NMR spectrum was acquired for each of all samples, employing the first increment of NOESY pulse sequence (recycle delay-90°- t1-90°- tm-90°-acquisition) with a spoil gradient for water presaturation.
Water suppression was achieved by chemical-shift-selective saturation, whereby three consecutive Hermite-shaped RF pulses, each of 15 ms duration, are applied followed by spoiling gradients preceding the PRESS sequence.
The MRA simulator implements a 3D Spoiled Gradient Echo sequence with optional flow compensation mechanism.
(42) The pulse sequence used a stimulated echo with bipolar gradient pulses and one spoil gradient, 43) followed by a 3 9 19 pulse for water suppression.
Three-dimensional structural MRI images were acquired on a 3.0 T GE Signa HDxt scanner (General Electric, Milwaukee, WI), using a T1-weighted fast Spoiled Gradient echo sequence.
A multiple flip-angle Spoiled Gradient Echo (SGE) imaging sequence was used to evaluate the intrinsic parameters maps M0 and T1 of the fruit tissues.
Firstly, we acquired a high resolution T1 weighted brain volume using a three dimensional inversion recovery spoiled gradient echo sequence (repetition time 97 ms, echo time 4 ms, inversion time 300 ms, sagittal acquisition).
First, we manually delineate the region of interest on a single breath hold fast spoiled gradient echo (FSPGR) sequence.
Volumetric images were acquired with a three-dimensional volumetric radiofrequency spoiled gradient echo pulse sequence 124 contiguous 1.2 mm sagittal slices (256 × 192 matrix, TE = 5 ms, α = 45, TR = 24 ms, NEX = 1, field of view = 30 cm).
