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For the Thin/NR, Thick/flat layers: 200 μL of the P3HT PCBM solution were placed onto either ZnO nanorod arrays or directly onto ITO, and after 7 s, it was spun at 600 rpm for 6 s, followed by a spin at 2,000 rpm for 60 s.
For the Thick/NR layers, 300 μL of the P3HT PCBM solution were spin-coated at 600 rpm (again waiting 7 s after dropping the solution) for 6 s onto the ZnO nanorod arrays followed by a spin at 1,000 rpm for 60 s.
Lysates were incubated for 20 minutes on ice, then cleared by a spin at 600 g for 3 minutes.
The 15%/60% cloudy interface was removed and washed twice with 10 ml of DMEM followed by a spin at 1500 r.p.m. for 5 min at 4 °C.
Reactions were incubated at various temperatures for 1 hr followed by a spin at max speed in a tabletop centrifuge for 10 min immediately prior to data collection to remove any trace aggregation.
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Despite having a 400-watt motor attached to it, the ZBoard rode just like a normal board when I took it for a spin at South by Southwest.
Deposition of LSMO was carried out by a spin coater at 500 rpm for 10 s, followed by 4,000 rpm for 50 s.
The resulting solution was deposited on the glass substrate (0.1 mL on a square of 2.5 × 2.5 cm2) by a spin coater at a rate of 3,000 rpm for 30 s, and then the as-deposited thin film was dried in a furnace at 350°C for 10 min to evaporate the solvent and remove organic residuals.
Fields as small as 3 nT can be detected in this way by using long averaging times (100 s) and spin echo pulsing tailored to decouple the NV spin from nearby C nuclei;(34) this is roughly equivalent to the field produced by a nuclear spin at a distance of 10 nm.
The suspended tissue was mixed thoroughly with a wide-bore pipette and filtered through two layers of Miracloth (CalBiochem, San Diego, CA) into an ice-cold 50-ml polyethylene centrifuge tube by a brief spin at less than 100 g for 5 s.
It was followed by a brief spin at room temperature.
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