We preferred the use of a fluid medium because the growth phases can be understood more easily than by culturing on a solid medium.
Nonlinear ultrasonic phenomena such as higher harmonics mode or mixed frequency response are represented by wave propagation through a solid medium (Payan et al. 2010; Van Den Abeele et al. 2000b).
Our study also showed that the replacement of water by a vacuum, rather than a solid medium, provides a better approximation of a water domain.
In this study, boronizing of 99.95% pure cobalt was performed in a solid medium by using Commercial LSB-II powders (that contain SiC) at 850, 900 and 950 °C for 2, 4, 6 and 8 h, respectively.
Antifungal activity was carried out by the agar incorporation method (dilution in a solid medium) including a negative control, as described previously by Bel Haj Salah et al. (2006) [ 19].
We calculated synthetic seismograms using the finite-difference method, assuming the following three models: the water domains treated as water (water model), the water replaced by a vacuum (vacuum model), and a solid medium (solid model).
Streptomyces mobaraensis, the wild-type strain (S. mobaraensis ECU7480, stored in our lab) and the mutants generated by ARTP mutagenesis, were grown on a solid medium comprising 20 g/L soluble starch, 3 g/L tryptone, 1 g/L KNO3, 0.5 g/L K2HPO4·3H2O, 0.5 g/L MgSO4·7H2O, 0.5 g/L NaCl, 0.01 g/L FeSO4·7H2O, and 1.5 2.0% (w/v) agar at pH 7.4 7.6 and 30 °C for 5 days.
A solid medium was prepared by mixing an equal volume of liquid medium at a 2× concentration with the same volume of (wt/vol) agar, 3.6% (Difco, Sparks, MD) that had been previously autoclaved.
All the colony numbers were obtained by the colony forming unit (CFU) method on a solid medium.
