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This suggests that the library diversity is greater than that sampled by a single sequencing run.
This may, thus, represent false positive RDA identification, represented by a single sequencing read (Table 1).
We have developed nanoCAGE to identify each transcript by a single sequencing read [ 1, 2].
The amplified beads were first run on a workflow analysis slide to determine the quality and quantity of the beads, which was followed by a single sequencing run, performed according to the Applied Biosystems SOLiD 4 System Instrument Operation Guide.
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When the number of blocks may be large, we propose efficient exact designs generated by a single sequence of treatment.
One a haplotype is found in 77 accessions and the other is represented by a single sequence of an accession (PI 210558) that was collected in China.
Nevertheless, it is not plausible to represent each species by a single sequence, especially for mytilid mussels from sunken whale carcasses and wood and shallow water, although many phylogenetic studies did so.
For the first three mutation positions, which have less than 100 bp distance from each other and can thus be covered by a single sequencing-read, no read showed more than one of the mutations simultaneously.
Until now, this morphospecies has been represented by a single sequence in the public databases (Z83958; 6).
In all, 94% of these species were represented by a single sequence.
The 5′GG/3′UU motif, which was previously represented by a single sequence, was added to the fitting.
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