Sentence examples for by a single restriction from inspiring English sources

Second, most mutations of interest occur outside the sequence cleaved by a single restriction enzyme.

Third, we streamlined restriction digestion by using only snip-SNPs that can be distinguished by a single restriction enzyme, DraI.

GBS utilizes multiplex sequencing of DNAs generated by a single restriction enzyme, ApeKI, a methylation insensitive restriction enzyme that recognizes the sequence GCWCG.

The different sub-clonings from the pCR-XL-Topo AtMRP6-GFP to the yeast vector pYES2 (Invitrogen®) and the mammalian expression vector pCI (Promega®) were realized by a single restriction with NotI.

Whereas the majority of outbreak-associated isolates (human clinical and food isolates) had an indistinguishable AscI PFGE pattern, one clinical isolate from Ontario and several isolates from the affected food production facility exhibited an AscI PFGE pattern that differed by a single restriction enzyme fragment compared to the primary outbreak strain.

This property has been used to design a strategy for sequential assembly of pre-made modules [12], or to replace the two steps of digestion and ligation by a single restriction-ligation step [13].

Identification of the Leishmania species studied was achieved by digestion with a single restriction endonuclease (RFLP), single-strand conformational polymorphism (SSCP) and DNA sequencing of the PCR-generated fragments.

Furthermore, PCR followed by a simple RFLP using a single restriction endonuclease was capable of identifying all Leishmania species commonly encountered in Greece.

First, we simplified SNP detection by using only SNPs that could be detected using a single restriction enzyme.

For lcp3, cutting with the restriction enzymes NotI and AscI (each has a single restriction site in lcp3), was confirmed by Southern blotting using an lcp3-specific probe.

Each selector targets a single restriction fragment and guides the circularization of this fragment by ligation, incorporating the general sequence motif into all fragments.