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Collectively, the case studies reveal that individual species usually are not threatened by a single, isolated factor.
How many big critical thinking problems of the world were solved single-handedly by a single, isolated human being?
Because extracted proteins electrophoresed on gel are necessarily modified or significantly degenerated, this approach may be fruitful only if the disease-specific nature, if detectable with silver-staining methods, is readily represented by a single isolated molecule even when it is modified for electrophoresis.
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Ten tc-positive Bt isolates (IBL 26, IBL 54, IBL 61, IBL 90, IBL 110, IBL 200, IBL 888, IBL 950, IBL 1115 and IBL 1140) belonged to ST 240, which until this report, had been represented by a single isolate in the PubMLST Bacillus cereus database [11].
In contrast, the 10 isolates of ST 240 in our sample are surprising considering that this sequence type is represented in the database by a single isolate of Bt toumanoffi exhibiting weak mosquitocidal activity against Culex spp. (Terrance Leighton and Katie Wheeler; unpublished data).
The 38 remaining STs were represented by a single isolate.
Fifteen MLGs were represented by a single isolate (Table 2).
Thirty STs were represented by a single isolate.
Twenty-three PFGE types were represented by a single isolate (singletons).
The remaining PFGE types were represented by a single isolate.
Serotype 1 was represented by a single isolate in 2006.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com