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In contrast, the 10 isolates of ST 240 in our sample are surprising considering that this sequence type is represented in the database by a single isolate of Bt toumanoffi exhibiting weak mosquitocidal activity against Culex spp. (Terrance Leighton and Katie Wheeler; unpublished data).
Ten tc-positive Bt isolates (IBL 26, IBL 54, IBL 61, IBL 90, IBL 110, IBL 200, IBL 888, IBL 950, IBL 1115 and IBL 1140) belonged to ST 240, which until this report, had been represented by a single isolate in the PubMLST Bacillus cereus database [11].
The 38 remaining STs were represented by a single isolate.
Fifteen MLGs were represented by a single isolate (Table 2).
Thirty STs were represented by a single isolate.
The remaining PFGE types were represented by a single isolate.
Similar(40)
There were 22 emm types that were represented by only a single isolate.
Tadinia was the first gene to be identified by controlled inoculation with a single isolate of Z. tritici, CA30 from California (Somasco et al., 1996).
At least two bacterial representatives of each pulsed-field gel type were further examined by using MLST (or a single isolate if this was the only representative of a given PFGE banding pattern type).
This recombinant strain, LEK122, was then subjected to EMS mutagenesis followed by adaptive evolution, resulting in a single isolate, LEK513, which displayed significantly improved xylose-utilizing property.
The DRC isolates formed the majority of Cluster A. Cluster B was dominated by isolates from Uganda, Kenya, and a single isolate from DRC, whereas Cluster C consisted of all of the isolates from Angola and single isolates from Germany, the Netherlands, Uganda, and DRC.
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