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The passage quoted just above in the main text suggests that dead force is related to living force by a single integration so that if dead force were as x, living force would be as ∫xdx (or, put conversely, that dead force is related to living force as its first derivative).
Genotoxicity caused by a single integration event mediated by the retrovirus-based vector has resulted in the development of T-cell leukemia in 5 of 20 patients treated for SCID with one death reported [ 39].
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The efficiency of transformation with the truncated vector was 20-fold lower in comparison to a non-truncated vector; this was likely caused by the selection for a single integration event (Skory 2005).
Galactose responsive constructs were constructed by amplification of the Gal1 promoter (defined as −1 to −625 nucleotides relative to the Gal1 ORF) from the yeast genome by PCR followed by restriction enzyme cloning into a single integration Trp1 or His3 marked vector upstream of Venus (YFP), yeGFP (GFP), or mKate2 (RFP).
With enhanced usage of A2 the chance of receiving functional GUS at a single integration site is increased by one third compared to exclusive usage of A1 emphasizing the potential of GT DsB for gene trapping.
We confirmed a single integration site of the Hermes retrotransposon by Southern blotting (Fig EV1B).
They could result from a single integration event, the phage DNA being duplicated by an uncharacterized DNA repair and/or replication event, or from multiple successive integration events.
Second, a single integration function is computed for each category.
Although these issues can be overcome by selection for ESC lines with single or low copy integrations and proper expression of the transgene [ 8], an approach that starts with a single integration into a predefined expression site is highly preferred.
Nearly 80%% of transformant GA activity varied within a range of 15 20 %, which could be explained by a single-copy integration of the GA fragment at the engineered locus.
Multiple copies could have arisen by multiple integrations of badnaviruses into the host genome or by the single integration of concatemers whose component sequences then diverged.
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