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Among the 339 TIC events, two-thirds (212) were supported by a single alignment and one-third (127) were supported by multiple alignments.
Regarding size of mitochondrial pseudogenes, we found six Numts ranging from 782 – 1,733 bp that were recovered by two or more alignments, and seven Numts of 131 – 412 bp recovered by a single alignment.
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Even the global tree was rejected at P < 0.05 by all but a single alignment; at P < 0.01, 15 markers did not reject this topology.
The SNPs are then concatenated into a single alignment by ignoring indels.
Joint estimation of alignment and phylogeny sidesteps biases that stem from conditioning on a single alignment by taking into account the ensemble of near-optimal alignments.
We start by highlighting the potential dangers of arbitrarily choosing protein models by demonstrating an empirical example where a single alignment can produce two topologically different and strongly supported phylogenies using two different arbitrarily-chosen amino acid substitution models.
If all overlapping transcripts yield consistent gene structures, they are assembled into a single alignment assembly, and by doing so, PASA acts to simply consolidate the transcript alignments into a single gene structure.
Multiple alignments for the same sample were combined by the use of BamTools (Barnett et al. 2011) into a single alignment.
The 1,197 protein sequence alignments were concatenated into a single alignment for phylogenetic inference.
Resulting alignments were then concatenated in a single alignment of 46,607 residues, in which missing proteins in some orthogroup alignments were replaced by tracks of gaps.
Then the two resulting alignments were combined into a single alignment using the "profile alignment" option of ClustalX [ 28].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com