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PCR products were cloned using TOPO TA cloning system (Invitrogen) and selected clones were sequenced from PCR products purified with Exonuclease I (USB) and Shrimp Alkaline Phosphatase (USB) on MegaBACE 500 automated sequencer or by a sequencing service (www.macrogen.com, last accessed February 7 , 2014.
Each string produced by a sequencing machine is referred to as a "read," and thousands of overlapping reads are created for every genome.
The absence of a signal peptide was verified by 5′ RACE PCR, followed by a sequencing of the resulting amplicon (341 bp).
This study proposed a promising strategy, electro-peroxone (E-peroxone) pretreatment followed by a sequencing batch reactor (SBR) for simulated hospital wastewater treatment, aiming to enhance the wastewater treatment performance and to reduce antibiotic resistance genes production simultaneously.
Their architecture was based on punched card equipment, but during each machine cycle (i.e., during the reading of a card, which took about half a second), the machine could execute a sequence of up to 48 steps, controlled by a sequencing device called a "hub". It was also possible to execute a "double cycle," with the feeding of the next card held until all operations were finished.
This is followed by a sequencing reaction with another nested primer.
Similar(30)
It was embodied by a sequence with eight minutes left.
Jarvis's ire was raised by a sequence late in the extra period.
I would represent the sequence 1, 2, 3 and 4 by a sequence of pairs.
"Any sound that the human ear can hear can be made by a sequence of digits.
They are cajoled, ticked off, hectored, humiliated and bullied by a sequence of advisers, trainers, bosses and team leaders.
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