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This analysis shows that ER-/PR+ breast cancer is by far the least reproducible breast cancer subtype, with the vast majority (94% and 97% in the two datasets) of cases classified as ER-/PR+ in the MR re-classified when testing was performed by a secondary method.
Nevertheless, interactions discovered using Y2H screens must be confirmed by a secondary method, such as co-affinity purification, to reduce the risk of false-positive interactions and to increase the confidence in the dataset, which is usually expected to reach >80% [ 20].
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Fisher's exact test was also applied as a secondary method, by setting a suitable threshold in the ranked list.
This can be remedied by combining the ROF method with a secondary method, like a seed based search algorithm.
Last year, Utah enacted a law that reinstated the firing squad as a secondary method should the state fail to obtain the drugs needed for a lethal injection.
The American Red Cross recommended it only as a secondary method to back-slapping.
In 1986, it was officially recommended as the primary anti-choking technique by the Red Cross, although the organization would reverse that decision in 2006, saying "abdominal thrusts" should only be a secondary method.
All unique CNVs (de novo and familial) were confirmed by an independent secondary method (such as FISH method) and only single copy gains or losses were identified.
The catalytic membranes were prepared by the ion-exchange of FAU (Na-Y) zeolitic membranes that were synthesized on tubular α-Al2O3 supports by a secondary growth method.
Ultrathin nanozeolite films were prepared on the surface of Pt and glass carbon electrodes using nanoscale zeolite particles as building block via a layer-by-layer (LbL) technique or by a secondary growth method.
The mordenite membrane was prepared on the outer surface of a porous alumina tubular support by a secondary-growth method.
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