Sentence examples for by a second wash from inspiring English sources

The gel pieces were subsequently washed in 40 µl of 30% ACN and 5% formic acid followed by a second wash containing 60% ACN and 0.1% formic acid.

Following incubation, slides were washed 1× for 3 minutes in 10×SSC, 0.2% Sarkosyl, followed by a second wash in 10×SSC for one minute.

Cell pellets were briefly washed twice for 5 minutes each in the phosphate buffered saline (PBS) to remove serum and other pertinacious material, followed by a second wash in Tris (hydroxymethyl)aminomethane.

Pellets were washed for 30 min in 50 mM sodium bicarbonate (pH 11.5) and centrifuged for 30 min at 45,000×g, followed by a second wash in 50 mM ammonium bicarbonate (pH 8.5) and further centrifugation for 30 min at 45,000×g.

Finally, the microarrays were washed once with 6× SSPE buffer (3.6 M NaCl, 0.2 M NaH2PO4, 0.02 M EDTA pH 7.4) containing 0.005% N-lauroylsarcosine for 1 min at room termperature followed by a second wash with preheated 0.06× SSPE buffer (37°C) containing 0.005% N-lauroylsarcosine for 1 min. The last washing step was performed with acetonitrile for 30 seconds.

Then, the preparations were washed with TBS for 10 minutes, followed by a second wash for a further 5 minutes.

After washing, a mouse monoclonal antibody against PGRN conjugated to horseradish peroxidase was added, followed by a second washing step and the addition of tetramethylbenzidine substrate.

Beads were pelleted followed by a second washing step with washing buffer II [20% (v/v) acetonitrile, 79.8% (v/v) water and 0.2% TFA].

Where biotinylated antibodies were used, secondary incubation with allophycocyanin-conjugated streptavidin (eBioscience) was performed and followed by a second washing before analysis.

The slides were rinsed with destilled H2O and after a 5 min incubation step in tris-buffered saline (washing buffer), endogenous peroxidase activity was blocked with DAKO Peroxidase blocking Reagent for 5 min followed by a second washing step.

After three washing steps for 5 minutes in PBS, the sections were incubated with the biotinylated secondary antibody (ABC Kit) for 1 hour at room temperature, followed by a second washing step.