Sentence examples for by a second strand from inspiring English sources

The Ambion Illumina RNA amplification kit uses the T7 oligo dT) primer to generate single-stranded cDNA followed by a second strand synthesis to generate double-stranded cDNA, which is then column purified.

This concept has been discussed by a second strand of the literature which proposes a single market with workers deciding where to work based on their own characteristics, wages, and benefits.

The involvement of a single strand of the attC suggests that the Holliday Junction (HJ) intermediate resulting from the recombination event cannot be resolved by a second strand exchange that would lead to the linearization of the replicon.

RiboZero depleted RNA was chemically fragmented, then made into cDNA with Superscript III (Life Technologies) and random hexamers followed by a second strand reaction.

The Ambion Illumina RNA amplification kit uses T7 oligo dT) primer to generate single stranded cDNA followed by a second strand synthesis to generate double stranded cDNA which is then column purified.

Reduced RNA was then reverse-transcribed to cDNA using random hexamers tagged with a T7 promoter sequence followed by a second strand cDNA synthesis using DNA polymerase (GeneChip WT cDNA Synthesis Kit, Affymetrix).

The researchers could pinch the tweezers shut by adding a fourth strand.

cDNA was prepared from total RNA by using a First Strand cDNA Synthesis kit (Roche, Indianapolis, IN).

Pre-formed RNA DNA hybrids were made by performing a first strand synthesis reaction on total RNA.

For the sequencing of cDNA total RNA was extracted from fresh-frozen tissue by using the Qiagen Minikit (Qiagen, Hilden, Germany), and then the total RNA was reverse transcribed by using a First Strand cDNA Synthesis Kit (Fermentas, St. Leon-Rot, Germany).

In total, 10 μg of total RNA spiked with poly-A controls (pGIBS-TRP, pGIBS-THR, and pGIBS-LYS; American Type Culture Collection) was converted to cDNA, using a T7 promoter-polyT primer (Affymetrix, Santa Clara, CA, USA) and the reverse transcriptase Superscript II (Invitrogen, Paisley, UK), followed by a second-strand cDNA synthesis (Invitrogen).