Sentence examples for by a second step from inspiring English sources

Ozone was used to remove most of the coke, followed by a second step with H2 or He.

The pellet was washed with 35 ml 1 PBS, followed by a second step of ultracentrifugation at 100,000 × g for 2 h at 4 °C.

The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare).

The exosomes pellet was washed with 24 ml PBS, followed by a second step of ultracentrifugation at 100,000 × g for 1.5 h at 4 °C.

The selected in-situ regeneration consists of entering firstly steam at relatively low temperature (< 250 °C), against most of published studies, followed by a second step with air.

To obtain stringent recovery of the antibodies, a two-step affinity chromatography principle was devised to first deplete the affinity tag-specific antibodies followed by a second step for affinity capture of the target protein-specific antibodies.

That day, Little-Brown began by playing a Second Step video featuring good friends, Lydia and Maria.

Prevent this by including a second step to access an account.

The separation process is further enhanced by introducing a second step.

20 GP practices were successfully reached by mail and 17 in a second step by follow-up telephone calls.

D* was then calculated in a second step by using an exhaustive search.