Sentence examples for by a second incubation from inspiring English sources

In an initial competitive experiment to exclude non-specific binding by FITC-Dex, cells were incubated with non-conjugated dexamethasone first, followed by a second incubation with FITC-Dex, which revealed a non-specific binding of less than 7%% (data not shown).

This was then incubated for 1 hour at 16°C, followed by a second incubation for 30 minutes at 20°C.

Infection was achieved by incubating at 37 °C for 30 min followed by a second incubation for 30 min at 37 °C and 250 rpm.

The eluted phage were added to 20 mL of the culture and incubated at 37 °C for 30 min followed by a second incubation for 30 min at 37 °C and 250 rpm.

The reaction was incubated at 65°C for 10 minutes in a thermocycler to disrupt secondary structures, followed by a second incubation on ice for at least 5 minutes.

Wool was equilibrated with 2% FCS in PBS, followed by a second incubation with 2% FCS in PBS at 37°C for 45 min. FCS/PBS fluid was eluted and afterwards spleen cell suspension was added.

In our experimental work here, we used an electrical heat block for this purpose; however, this could be accomplished with the electricity-free heater by the inclusion of a parallel heating unit at a higher temperature (essentially, by including a second incubation chamber that uses a different EPCM, or no EPCM at all).

Gq/11 KO MEFs and their WT parents as well as the β-arrestin-1 and -2 downregulated GT1-7 lines (712 and 153) and their WT control GT1-7 cells were serum-starved overnight followed by a wash in HBSS and a second incubation in serum-free media for one hour.

These are the rhythms of an extraordinary, and extraordinarily demanding, time in the life of the photographer's family — a second incubation of sorts, animated by the entanglements of necessity and joy.

Consequently, this system is activated via the classical pathway of complement activation only by those antibodies which, in a first incubation step, have been bound to their target molecules on the donors' cells.

After an additional washing step in TBS-TX, a pre-incubation of 10 min in TBS-TX with 0.4%% ammonium nickel sulphate (Sigma, St . Louis MO, USA) was performed, followed by a second pre-incubation of 10 min in TBS-TX with 0.4%% ammonium nickel sulphate and 0.015 % 3,3-diaminobenzidine (DAB; Sigma, Buchs, Switzerland).