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The evidence is consistent with a model that proposes an initial duplication of a B- Myb-like ancestral gene, followed by the evolution of a central transcriptional activation in one of the duplicates, followed by a second duplication of the proto-c/A- Myb gene to generate the c- Myb and A- Myb genes of existing vertebrates (Davidson et al., 2005).
This would be followed by a second duplication event in which Npi37-2-pre was duplicated to produce Npi37-2 and Pish.
It is possible that the vertebrate gene family arose as a duplication of APLP-1 followed by a second duplication to form APLP-2 and AβPP.
The second scenario involves duplication of the RecJ proper homolog (ancestor of arCOG00427) at the base of archaea with several independent losses of arCOG00427_I followed by a second duplication in Euryarchaeota yielding arCOG00429.
Incongruence between the NRPD2/E2-like gene phylogeny and species phylogeny indicates a first duplication of NRPD2 relatively basally in Violaceae, with subsequent sorting of paralogs in the descendants, followed by a second duplication in the common ancestor of Viola and Allexis.
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(4) A second duplication occurred recently in Gadus.
A second duplication event resulted in GR1 and GR2 within the second major clade of bony fish, the actinopterygian/ray-finned fishes (represented by trout, cichlids and midshipman).
Of the three PI5P4K isoforms, the earliest divergence is apparently that of PI5P4Kγ resulting from a gene duplication event with a later divergence of PI5P4Kα and PI5P4Kβ by a second gene duplication, suggesting that these two isoforms have a closer evolutionary relationship.
The current paradigm of WGD predicts a first round of duplication after the emergence of vertebrates but before the separation between agnathans and gnathostomes, followed by a second round of duplication after this divergence [ 34].
A-Myb and c-Myb appear to have arisen by a second round of gene duplication, which was preceded by the acquisition of a transcriptional activation domain in the ancestral A-Myb/c-Myb gene generated from the initial duplication of an ancestral B-Myb-like gene.
The presence and localisation of the duplication was confirmed by a second method, reinforcing the fact that this duplication was not an artefact caused by the presence of CHEK2 pseudogenes [ 22].
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