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A PSA response is defined as a decrease of serum PSA levels by at least 50% compared to baseline and confirmed by a second determination 4 weeks later.
Prostate specific antigen (PSA) values were measured following surgery and PSA recurrence was defined as a postoperative PSA of ≥ 0.2 ng/ml confirmed by a second determination with a serum PSA ≥ 0.2 ng/ml.
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A second determination of DCF was obtained directly from the fraction collected by the Effective Dosimeter.
A second determination on the 15 isolates confirmed the MICs.
This was confirmed by a second independent replication peak determination, in which average distance between bona fide origins found in both experiments was 6.9 ± 2.4 kb (Additional file 4).
ELISA and ECL samples found positive in screening were verified by a second confirmatory assay and titre determination.
For all analyses, a second, confirmatory determination was performed.
To assess reliability of AE determination, 10% of the second phase records are reviewed independently by a second specialist.
For this study, although all clarity determinations were made by a single observer, there was no confirmation by a second observer.
Subsequently motivations to attend were assigned along the self-determination continuum [ 20] by a single researcher (FB or NB) and this was checked by a second researcher (VN).
11 In case of divergent polarising microscopy results, the specimen was reviewed by a third investigator who made the final determination regarding the presence of MSU crystals.
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