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The protected azido-folate 4 was purified in two steps: first by aluminum oxide column chromatography, followed by a second column based on silica gel.
Fraction 2 (300 mg) was subjected to column chromatography on silica gel with pentane diethyl ether (3:2) and further purified by a second column chromatographic separation with pentane ethyl acetate (7 3) to give 3 mg of stigmasta-5,28-dien-3β-ol stigmasta-5,28-dien-3β-ol stigmasta-5,28-dien-3β-ol stigmasta-5,28-dien-3β-ol stigmasta-5,28-dien-3β-ol
The crude extract (300 mg) was subjected to column chromatography on silica gel with a mixture of pentane diethyl ether (4:1) to give 19 mg of a product mixture which was further purified by a second column chromatography using the same conditions to afford 13 mg of fucosterol (3).
For example, in three-band division of four-column transform matrix, the band b 1 is determined by a first column sequency, the band b 2 is determined by a second column sequency, and the third and fourth columns generate band b 3.
Similar(56)
The dual sampling approach is the method of improving the sensitivity by adding a second column signal processing chain circuit and a capacitor bank to a conventional architecture of CMOS image sensor without modifying the data readout method [11].
Offset mismatches of the source follower are also corrected by a second column-level DS stage.
In second stage CD25+ was stained with PE-labeled monoclonal antibodies, mixed with anti-PE magnetic microbeads and positively selected by passage through a second column under a magnetic field.
A relief force was stopped by the Boers, while a second column turned back believing that the post had already been relieved.
Yesterday's executive inaction will be followed today by newspaper advertisements by YES that will feature, in one column, a list of the 25 cable operators that are carrying YES on basic tiers, and in a second column, Cablevision by itself.
Their efforts, hélas, were ultimately undermined by a fifth column: France's own industrial chocolate makers.
Virtually surrounded by four columns of Francoist troops, the city was plagued by a fifth column of snipers and saboteurs.
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