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A test of the conclusion that the DNA in the intermediate density band was half labeled was provided by examination of a sample containing equal amounts of generations 0 and 1.9.
This feature was exploited to allow the single-step enrichment by 100 000-fold of a sample containing just one positive cell per 1 000 000 negative cells.
The most sensitive detection was by the Microsens enzyme immunoassay with a sample containing 12 μg vCJD tonsil tissue; the equivalent for the Bio-Rad enzyme immunoassay was 480 μg vCJD tonsil tissue (table 1).
We confirmed this conclusion by titrating CpxI(47-134) itself on a sample containing SCΔ60 and 1.5 equivalents of CpxI(47-134), which logically yielded data similar to those observed in the direct titration experiment of Figure 4B beyond 1.5 equivalents.
Previously, scientists had to understand distinct qualities of proteins by measuring a sample containing a huge number of molecules.
Molecular mass and pI values were estimated by running a sample containing serum proteins together with a mixture of protein standards (Bio-Rad).
Their stability was tested by 20 randomisations, each one providing a sample containing half of patients, and by repeating HCA on each sample; cases that did not remain in the same cluster on two HCA were considered unclassifiable [21].
Measurement assurance for genome-scale measurements is challenged by the impracticality of creating a sample containing known quantities of tens of thousands of components, such as the RNA transcripts measured in an RNA-seq experiment.
By admixing a FFPE sample containing a variant with a FFPE sample containing no mutant, we were able to determine that an allele frequency of about 5% was still detectable when the mean read depth was >1,000x.
The Er content was obtained by time-of-flight secondary ion mass spectroscopy technique after calibration by a reference SRSO Er sample containing a known Er concentration.
For a typical ELISA process, a capture anti-cTnI antibody against cTnI is firstly immobilized onto the surface of plastic well, followed by adding a patient sample containing cTnI for binding the capture anti-cTnI antibody.
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