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Recovery and precision of the method, as well as the standard calibration curves, were determined by a routine method [ 24].
Serum creatinine was measured by a routine method in the Laboratory of Clinical Chemistry, University Hospital, Umeå, Sweden.
Sections cut at 6 μm were dewaxed and subjected to immunofluorescence staining and one section was stained by a routine method (HAS) containing haematoxylin, azofloxin and saffron.
Fluid collected on the swab from the affected area was transferred onto a glass slide for Gram staining by a routine method.
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Detection of p53 protein by immunohistochemistry has become a routine method in pathology practice, and the presence of detectable p53 protein is an important prognostic marker that correlates with higher histopathological grade, increased mitotic activity, aggressive tumour behaviour and therefore a worse prognosis [ 15, 16].
Large-scale gene-expression (GEX) profiling by DNA microarrays has become a routine method in breast cancer research.
Gene silencing by RNA interference (RNAi) has become a routine method for extracting function from the mammalian genome.
Reduced glutathione (GSH) was assessed by a routine colorimetric method with Ellman's reagent.
Total serum IgA levels were determined by a routine turbidimetric method.
Urinary creatinine was measured by a routine chemistry method and used for calculation of creatinine-corrected urinary CTX-II concentrations.
Glycemia and Lipid Profile were immediately quantified by a routine colorimetric method (Biosystems BTS-303 Photometric, Spain).
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