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The stability was not affected by a reducing condition, freeze thawing for up to 30 cycles, or lyophilization.
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However, because they each represent single polypeptide chains, their electrophoretic mobility was not affected by reducing condition except that a trailing smudge preceded the major stained bands of approximately 63 kD, and 96 kD, respectively.
Autoradiographic analysis of CD98 immunoprecipitate resolved by SDS-PAGE under reducing condition revealed an 85-kDa phosphorylated band that represents glycosylated CD98 (Fig. 5A, left panel).
Free Png1p in the cytosol may deglycosylate the glycoprotein fragments generated by proteolysis or unfolded glycoproteins by reducing conditions maintained in cytosol.
Whole cell lysate was added to 5× electrophoresis sample buffer and separated by SDS/PAGE under reducing conditions on a 12% separation gel.
Proteins were then separated by SDS/PAGE under reducing conditions on a 12% gel and transferred to PVDF membranes.
Reducing condition was maintained by adding 5% β-mercaptoethanol to the SDS-load buffer.
Reductive dechlorination of tetrachloroethene (PCE) and its daughter products in aquifers is often hampered by Fe III) reducing conditions.
For the precursor with high nickel support interaction, both the degree of reduction and the final catalyst metallic area were strongly affected by the reducing conditions.
To test if its functionality had been damaged by the reducing conditions used during EPL it was intensively analyzed.
Analysis were undertaken to test if the antibody had been damaged by the reducing conditions used during PTS.
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